Effects of lead exposure on the activity of erythrocyte pyrimidine 5'-nucleotidase and delta-aminolevulinic acid dehydratase in mice.
نویسنده
چکیده
T0M0KUNI, K. Effects of Lead Exposure on the Activity of Erythrocyte Pyrimidine 5'-Nucleotidase and s-Aminolevulinic Acid Dehydratase in Mice. Tohoku J. exp. Med., 1983, 139 (1), 27-32 The effects of lead exposure on the activities of erythrocyte pyrimidine 5'-nucleotidase (Py5N) and s-aminolevulinic acid dehydratase (ALA-D) were investigated in the mice given ad libitum the drinking water containing lead at 10 to 500 ppm for 30 days. The erythrocyte Py5N activity was significantly inhibited in the 250 and 500 ppm groups, and the activity in these groups was dropped to 62-81% of that in the control group. In addition, the erythrocyte Py5N activity was shown to have a significant negative correlation with the concentration of lead in blood (r= -0.79). The erythrocyte Py5N activity would be a better indicator than ALA-D in the exposure to relatively high lead concentrations, although the assay of Py5N is more time-consuming as compared with that of ALA-D, while, the erythrocyte ALA-D activity is useful in the evaluation of exposure to low levels of lead. The erythrocyte Py5N was less stable than ALA-D against heating at 60°C. lead; erythrocyte; pyrimidine 5'-nucleotidase; s-aminolevulinic acid dehydratase
منابع مشابه
Evaluation of lead exposure in workers at a lead-acid battery factory in Korea: with focus on activity of erythrocyte pyrimidine 5'-nucleotidase (P5N).
OBJECTIVE To evaluate lead exposure among lead-acid battery workers in Korea, to evaluate in more detail the erythrocyte pyrimidine 5'-nucleotidase (P5N) test for lead exposure, and to evaluate the abnormal accumulation of erythrocyte pyrimidine nucleotides in the battery workers. METHODS Activity of P5N and other biological variables were examined in 66 exposed workers in a lead-acid battery...
متن کاملA simplified method for determining erythrocyte pyrimidine 5'-nucleotidase (P5N) activity by HPLC and its value in monitoring lead exposure.
The method for determining erythrocyte pyrimidine 5'-nucleotidase (P5N EC 3.1.3.5) activity has been simplified using an automated high performance liquid chromatograph (HPLC). The activity determined by the simplified method agreed closely with that obtained by conventional methods. In 161 lead workers P5N activity declined linearly with increasing blood lead concentrations (Pb-B) between 20 a...
متن کاملEffects of low-level lead exposure on pyrimidine 5'-nucleotidase and other erythrocyte enzymes. Possible role of pyrimidine 5'-nucleotidase in the pathogenesis of lead-induced anemia.
Similarities between lead-induced anemia and a new hereditary erythorenzymopathy involving pyrimidine-specific 5'-nucleotidase prompted studies of the effects of lead on this and other erythrocyte enzymes. In vitro incubations of normal mature erythrocytes demonstrated that significant inhibition of pyrimidine 5'-nucleotidase occurred in the presence of lead at concentrations that had minimal e...
متن کاملErythrocyte delta-aminolaevulinic acid dehydratase activity and blood protoporphyrin concentrations as indices of lead exposure and altered haem biosynthesis.
1. The activity of erythrocyte 6aminolaevulinic acid (ALA) dehydratase and blood protoporphyrin concentrations have been measured in patients with various anaemias, a group of subjects with known lead exposure and a group of control subjects. Leucocyte ALA synthase was measured in subjects from the last two groups. 2. Erythrocyte ALA dehydratase activity was significantly depressed in the group...
متن کاملLead toxicity in the pregnant rat. II. Effects of low-level lead on delta-aminolevulinic acid dehydratase activity in maternal and fetal blood or tissue.
Lead was administered to pregnant rats in drinking water (5 ppm) from day 1 to day 18 or 21 of gestation, at which time the dams and fetuses were sacrificed. The red blood cell count and hemoglobin concentration were significantly lower in the lead-treated fetuses than in untreated controls on day 21 of gestation, but not in the lead-treated dams. The delta-aminolevulinic acid dehydratase (ALAD...
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ورودعنوان ژورنال:
- The Tohoku journal of experimental medicine
دوره 139 1 شماره
صفحات -
تاریخ انتشار 1983